MICROBIAL ADHESION OF CRYPTOSPORIDIUM PARVUM SPOROZOITES: PURIFICATION OF AN INHIBITORY LIPID FROM BOVINE MUCOSA
نویسندگان
چکیده
منابع مشابه
Infectivity and neutralization of Cryptosporidium parvum sporozoites.
Cryptosporidiosis, a diarrheal disease of calves and humans caused by the coccidian parasite Cryptosporidium parvum, is terminated in hosts with normal immune systems. To assess the mechanisms of immunity in cryptosporidiosis, it is necessary to isolate and quantitate sporozoites, the infective stage of Cryptosporidium spp. Here we report the (i) separation of infective C. parvum oocysts from c...
متن کاملAttachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells.
An enzyme-linked immunosorbent assay-based attachment model using the human intestinal cell line Caco-2A was developed to study attachment of Cryptosporidium parvum sporozoites in vitro and to assess potential inhibitors of sporozoite binding. In this system, attachment was related to sporozoite dose, incubation time, and host cell differentiation status. Polyclonal antibodies to C. parvum as w...
متن کاملImmunolocalization of an enterotoxic glycoprotein exoantigen on the secretory organelles of Cryptosporidium parvum sporozoites.
In this study, the fine ultrastructures of the secretory organelles of C. parvum sporozoites were demonstrated using transmission electron microscopy (TEM). Meanwhile, a previously identified enterotoxic 18-20 kDa copro-antigen (18-20 kDa CCA), associated with cryptosporidiosis in both human and calves, was isolated and immunolocalized on C. parvum sporozoites. Using immunoelectron microscopy a...
متن کاملCharacterization of an immunogenic glycocalyx on the surfaces of Cryptosporidium parvum oocysts and sporozoites.
Ruthenium red staining of Cryptosporidium parvum oocysts revealed the presence of a carbohydrate matrix on their outer bilayers that is characteristic of a glycocalyx. Surface labeling of intact oocysts identified material of high molecular weight (>10(6)) that reacted positively with sera from cryptosporidium-infected patients and with immunoglobulin A monoclonal antibodies.
متن کاملPathogenesis of human and bovine Cryptosporidium parvum in gnotobiotic pigs.
To compare the pathogenesis of human genotype 1 (HuG1) and bovine genotype 2 (BoG2) Cryptosporidium parvum, neonatal gnotobiotic pigs were given 1-10 HuG1 or BoG2 oocysts. The prepatent and patent periods were significantly longer for HuG1 than for BoG2 C. parvum (prepatent, 8.6 vs. 5.6 days; patent, 16.6 vs. 10.3 days). BoG2-infected pigs developed significantly more severe disease than did Hu...
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ژورنال
عنوان ژورنال: Journal of Parasitology
سال: 2004
ISSN: 0022-3395,1937-2345
DOI: 10.1645/ge-231r